TECHNOLOGY AND PRODUCTION

Antimicrobial activity of squalene and CO2 extracts of amaranth seeds

Authors

  • Anna A. Petrova Kemerovo State University, 6, Krasnaya st., Kemerovo, 650000, Russia
  • Olesya V. Salishcheva Kemerovo State University, 6, Krasnaya st., Kemerovo, 650000, Russia
  • Vladimir P. Yustratov Kemerovo State University, 6, Krasnaya st., Kemerovo, 650000, Russia
  • Timofey A. Larichev Kemerovo State University, 6, Krasnaya st., Kemerovo, 650000, Russia

How to cite

GOST Petrova A. A., Salishcheva O. V., Yustratov V. P., Larichev T. A. Antimicrobial activity of squalene and CO2 extracts of amaranth seeds // Bakery of Russia. 2025. Vol. 69. No. 1-2. P. 10-20.
APA Petrova, A. A., Salishcheva, O. V., Yustratov, V. P. & Larichev, T. A. (2025). Antimicrobial activity of squalene and CO2 extracts of amaranth seeds. Bakery of Russia, 69(1-2), 10-20.

Abstract

Vegetable oils exhibit a variety of antimicrobial properties, which confirms their value as a natural resource. The aim of the study was to determine the antimicrobial activity of squalene and CO2 extracts of amaranth. Antimicrobial activity was assessed against test cultures of Escherichia coli, Pseudomonas aeruginosa, Pseudomonas putida, Enterococcus faecalis using the disk diffusion method. The studies were carried out for three extracts of amaranth oil obtained by superfluidic CO2 extraction, extracted at various pressures (100, 160, 290 atm.), as well as for squalene isolated by multistage hexane extraction. An antibiotic solution and water were used as positive and negative controls. It was found that all extracts have antimicrobial activity, but the extract obtained at 290 atm shows higher antimicrobial activity against the following strains of microorganisms Escherichia coli, Pseudomonas aeruginosa and Pseudomonas putida. This can be seen from the zones of inhibition of microbial growth. At high pressure, the dissolving capacity of CO2 is higher than at pressures of 100 atm and 160 atm, therefore, this leads to an increased concentration of precisely those biologically active substances that have an inhibitory effect on microorganisms. The obtained extracts have demonstrated antimicrobial potential, which indicates the possibility of their use as environmentally friendly antimicrobial systems. These results may be useful for the development of antimicrobial complexes for potential use in biomedical, pharmaceutical and food systems.

Keywords

amaranth antimicrobial activity CO2 extracts squalene microorganisms

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Issue

Vol. 69 No. 1-2 (2025): Bakery of Russia

Section

TECHNOLOGY AND PRODUCTION

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